Antibody drug with four drug compounds linked to IgG immunoglobulin

High Mass MALDI Analysis

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This service provides the molecular weight of a protein sample up to 2 MegaDalton. It can be done on the intact protein or even with intact protein complexes. Because of our unique technology of high mass MALDI detection at CovalX, we routinely measure the mass of intact molecules of several hundred kDa or even into the MegaDalton range. This is a technology invented by CovalX and CovalX is the exclusive CRO service provider using this high mass technology. See more information about high mass detection system here.

CovalX High Mass MALDI Spectra_IgM High-Mass MALDI ToF mass spectra of intact IgM hexamer. IgM can be present as a pentamer (approx 950kDa) or hexamer (as shown). These differences can be directly detected with the CovalX high mass MALDI detection.

CovalX utilizes the latest mass spectrometry instrumentation for Matrix-Assisted Laser Desorption Ionization (MALDI) mass spec analysis. Our instruments can identify ions in both positive and negative ion mode if required. In our CRO service laboratory, we have several high mass MALDI-TOF instruments allowing singly charged ion detection up to 2 MDa.

Although we prefer samples to be of the highest purity and concentrations possible, MALDI is a more tolerant technique for “dirty” or difficult samples. Our typical analysis involves a dilutions series of measurements with pre-cleanup steps possible as well.

This high mass MALDI technology is regularly used for characterization of very large molecules such as IgM which can be found as a pentamer (950 kDa) or as a hexamer (1.2 MDa). The CovalX high mass MALDI technology provides one of the only methods to rapidly and directly discern between these different states of the molecule. Additionally, this technology can be used to look at total deglycosylation content or other stripping of these large molecules. Countless other large molecules such as monoclonal antibodies, modified (ADC, PEGylated) antibodies, smaller VLPs or AAVs have been measured directly with this method.

Additionally, many intact protein complexes are routinely characterized using the CovalX high mass MALDI services including mAb complexes, KLH, COVID S2 spike protein trimers, aggregates and many other protein interactions.

If you have a large molecule or protein complex which you would like to analyze please feel free to contact us.

Sample types: Recombinant proteins, purified proteins

Data turn around: Two weeks or less

Why Choose CovalX’s High Mass MALDI Analysis Service?

Expertise: Experienced epitope mapping team servicing the industry for more than 15 years. Sample handling is conducted using the latest automation robotics.

Quality Work: Unique initial intact screening of proteins (and protein complexes) by high mass MALDI provides the most reliable rate of success. Efficient processing is conducted using its design of experiment (DOE) software.

Sample types: Recombinant proteins, purified proteins

Data turn around: Two weeks or less

If you have a large molecule or protein complex which you would like to analyze please feel free to contact us.